Coding

Part:BBa_K2029005:Design

Designed by: Max Schelski, Niklas Schmacke   Group: iGEM16_UBonn_HBRS   (2016-10-14)


EngB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part codes for endoglucanase engB from Clostridium cellulovorans. It has been expressed and secreted in Bacillus subtilis strain WB800 using the secretion tag of sacB behind the P43 promoter in plasmid pWB980 (KanR). (ref 1)


Source

Clostridium cellulovorans

References

1 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1785254/